SARS-CoV-2 vaccination and semen quality: a study based on sperm donor candidate data in southwest China.

Background: The coronavirus disease 2019 (COVID-19) pandemic has been a global health crisis and continues to pose risk to population health at the present. Vaccination against this disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has become a public health priority worldwide. Yet, limited information is available on the potential impact of such vaccines on human fertility. Methods: To examine the relationship between COVID-19 vaccination and male fertility, we conducted an observational study on sperm donor candidates in China who received Chinese COVID-19 vaccines between January 1, 2020 to December 31, 2021. Results: A total of 2,955 semen samples from 564 individuals were assessed along with vaccination information. Statistical analyses were conducted on both the entire study population and the subgroup of individuals who provided repeated semen samples before and after vaccination. While motility related parameters [progressive rate, curvilinear velocity (VCL), average path velocity (VAP), straight-line velocity (VSL), wobble (WOB), straightness (STR), linearity (LIN), amplitude of lateral head displacement (ALH), beat-cross frequency (BCF)] exhibited statistically significant difference before and after vaccination based on Welch two-sample test, mixed effects regression results based on repeated measures from the same individuals indicated that vaccination was not statistically associated with sperm quality parameters except for VCL, VAP, and VSL. Individual variability was the key determinant of sperm quality variance, with contribution ranging from 19% to 82%. Conclusions: Findings from our study could help to enhance current understanding of male reproductive health in the context of the global pandemic.

Utilization of phosphoric acid-modified biochar to reduce vanadium leaching potential and bioavailability in soil.

Remediating vanadium (V) polluted soil has garnered widespread attention over the past decade. Yet, few research projects have investigated the stabilization of soil V using modified biochar, so the effects and interacting mechanisms between soil properties and modified biochar for V immobilization and stabilization remain unclear. Hence, this gap is addressed by determining the leaching behavior and mechanisms of soil V on different dosages of phosphoric acid (H3PO4) impregnated biochar (MLBC, 0.5%-4%). The applicability and durability in soil V immobilization was investigated under acid precipitation. The MLBC effect on V bioavailability and mobility was assessed first by CaCl2, Toxicity Characteristic Leaching Procedure (TCLP) and Synthetic Precipitation Leaching Procedure (SPLP) extractions in different periods. The V concentrations significantly reduced in CaCl2, TCLP, and SPLP extract with MLBC at each dosage (30 d), while slight to significant increase in SPLP and TCLP extract V was recorded in a long-term incubation (90 d). Column leaching test further demonstrated the high durability of 4% MLBC in V stabilization under continuous acid exposure. Compared to the control (no-biochar), the accumulated V content in the leaching solution significantly decreased in MLBC-amended soil. Acid soluble fraction of V showed significant negative correlation with both soil organic matter (SOM) and available P, which was positively correlated with pH, suggested that pH, available P and SOM were key factors affecting the bioavailability of V in soil. Moreover, combining with the characterization results of MLBC and amended soil, the results revealed that H3PO4 modified biochar played a vital role on V immobilization and soil improvement by forming electrostatic adsorption, ion exchange, redox reaction or complexation with the increase of functional groups. These revealed an efficient and steady development of soil quality and treatment for soil V contamination, under MLBC operation to soil polluted with exogenous V.

Transplanted Ginkgo growth rates indicate common Chinese nursery techniques may severely limit urban ecosystem services.

China has experienced history's largest rural-to-urban migration. The social, economic, and environmental challenges brought about by urbanization are diverse and complex. Given China's national goal to achieve carbon neutrality by 2060 and commitment to urban sustainability, large cities have focused on urban greening initiatives. Yet, studies seeking to quantify ecosystem services and disservices only assess healthy, mature trees, rather than those with severe damage, declining health, or lack of vitality due to poor management. In this short communication, we conducted a case-study in one of China's major nursery stock-producing cities, Chengdu, on a common street tree, Ginkgo biloba, to assess the long-term impact of one of the most common yet extreme nursery transplant practices on tree growth (traumatic root-cutting of 'super-large' nursery stock). We used tree-ring data collected in a typical urban greenspace from 23 Ginkgo trees, including 18 trees transplanted as 'super-large' nursery stock and a control group (5 trees) transplanted as small-caliper trees. We found the trees transplanted as 'super-large' nursery stock experienced declining tree growth with decades of lost landscape potential likely due to traumatic root-cutting at the time of transplant from nursery to landscape. The control group allowed contrast between the growth patterns of 'super-large' transplanted trees with those that remained healthy, being transplanted as smaller-caliper trees. For the 'super-large' trees, we found a decrease in carbon sequestration from 7.6 kg C yr-1 on average per tree in 2001 to about 1.5 kg C yr-1 on average per tree in 2021, while no decreasing trends were observed among the control trees. This implies a negative impact on multiple expected ecosystem services including carbon sequestration, shade, canopy coverage, and pollutant mitigation. These results highlight the unrecognized costs of common Chinese nursery and transplant techniques on urban landscape trees, necessitating more research, science-based policies, and better management techniques.

Assessing the impact of urban greenspace on physical health: An empirical study from Southwest China.

Introduction: As the world becomes increasingly urbanized and human-nature contact declines, urban greenspace's impact on human health has garnered growing interest across academic disciplines. Various definitions and multiple indicators of greenspace have been utilized, with most studies finding an overall positive association between greenspace and health. Nevertheless, studies directly comparing how different greenspace indicators impact different disease types have been limited. Moreover, to verify the robustness of conclusions drawn, studies should compare multiple measures of greenspace across various spatial scales. Thus, a more comprehensive analysis is necessary to help inform future study design, especially in determining which greenspace indicators would be most useful in data-limited areas. Methods: Chengdu, the capital city of Sichuan Province, is West China's largest and most urban city, being typical of other large cities in lower to middle-income countries (LMICs). With twenty county-level jurisdictions spanning various degrees of urbanization, Chengdu's landscape heterogeneity and large population make it ideal for studying greenspace's impact on public health. This study took Chengdu as a case study to assess the association and potential impact of three traditional measures of greenspace (Normalized Difference Vegetation Index, Enhanced Vegetation Index, and Fractional Vegetation Cover) and urban ratio (% of population being urban) on hospitalization rates and medical expenses paid for three major disease categories (circulatory system diseases, neoplasms, and respiratory system diseases). Results and discussion: We found greenspace did have a significant impact on public health, but this relationship differed by disease type. Greenspace exhibited significant positive association with respiratory diseases, but insignificant negative associations with the other disease categories. Urban ratio showed significant negative association with greenspace abundance. The higher the urban ratio (e.g., less greenspace), the more money was paid on medical expenses. This relationship was found not only in terms of urban ratio being positively correlated with medical expenses, but also in that all three greenspace indicators were negatively correlated with medical expenses. Consequently, in future health outcome studies, urban ratio could be an acceptable negative indicator of greenness in LMICs where urban ratio is likely to imply less greenness.

Declining tree growth rates despite increasing water-use efficiency under elevated CO2 reveals a possible global overestimation of CO2 fertilization effect.

Though rising atmospheric CO2 concentrations (Ca) harm the environment and society, they may also raise photosynthetic rates and enhance intrinsic water-use efficiency (iWUE). Numerous short-term studies have investigated tree growth under elevated CO2 (eCO2) conditions, but no long-duration study has investigated eCO2 impacts on tree growth and iWUE under natural conditions. Utilizing a new dendrochronological experimental design in a heavily-touristed nature preserve in Southwest China (Jiuzhaigou National Nature Reserve), we compared tree growth (e.g., basal area increment) and iWUE in two biophysically and environmentally similar valleys with contrasting anthropogenic activities. Trees in the control valley with ambient CO2 benefited from increasing Ca, possibly due to the CO2 fertilization effect and optimal environmental conditions. However, trees in the treatment valley with intensive tourism experienced comparatively higher localized eCO2 and growth rate declines. While iWUE increased (1959-2017) in the control (25.3%) and treatment sites (47.8%), declining tree growth rates in the treatment site was likely because comparatively extreme CO2 exposure levels encouraged stomatal closures. As the first long-term study investigating eCO2 impacts on tree growth and iWUE under natural conditions, we demonstrate that increased forest iWUE is unlikely to overcome negative drought stress and rising temperature impacts. Thus, forest potential for mitigating eCO2 and global climate change is likely overestimated, particularly under dry temperate conditions.

Comparing KTP and CO2 laser excision for recurrent respiratory papillomatosis: A systematic review.

Objective: The CO2 laser and 532 nm potassium titanyl phosphate (KTP) laser have been applied to treat recurrent respiratory papillomatosis (RRP). This systematic review sought to compare outcome differences between these two methods. Data Sources: Embase, Web of Science, PubMed, and the Cochrane Library. Review Methods: CO2 laser and KTP laser studies were obtained by keyword searches of four authoritative medical databases. Articles were screened and retained when conforming to inclusion criteria. The primary outcome was cure rate; the secondary outcomes were recurrence, death, remission, clearance, and human papillomavirus (HPV)-detected rates, as well as laser effectiveness rates. Postoperative complications rate was the safety outcome measure. All outcomes were summarized within the CO2 and KTP groups, with results statistically compared (p < .05). Results: Overall, the cure rates were 87.25% (KTP group) and 75.98% (CO2 group; p < .05). Complication rates significantly differed between the KTP (2.32%) and CO2 (17.71%) groups (p < .0001). There was a relatively higher but not significant difference in the recurrence rates between the CO2 (18.6%) and KTP (10.87%) groups (p = .1595). The CO2 group remission rate was considerably lower (38.9%) than the KTP group (88.46%, p < .0001). HPV-detected and clearance rates were only reported for the CO2 group. The bias risks were 13.1 ± 1.45 (CO2) and 13.6 ± 1.52 (KTP) for the two groups, indicating evidence was of fair quality. Conclusion: Overall, KTP laser excision showed significantly better postoperative clinical outcomes than the CO2 laser, with a lower failure rate. Available fair-quality evidence suggests KTP laser excision might be better for treating RRP. Nevertheless, more high-quality randomized controlled studies are needed to compare these two surgical techniques, particularly in terms of reporting functional data such as vocal outcomes.

An External Patient Healthcare Index (EPHI) for Simulating Spatial Tendencies in Healthcare Seeking Behavior.

Background: Healthcare resources are always more limited compared with demand, but better matching supply with demand can improve overall resource efficiency. In countries like China where patients are free to choose healthcare facilities, over-utilization and under-utilization of healthcare resources co-exist because of unreasonable healthcare seeking behavior. However, scholarship regarding the spatial distribution of utilization for healthcare resources, resulting from unreasonable spatial tendencies in healthcare seeking, is rare. Methods: In this article, we propose a new External Patient Healthcare Index (EPHI) to simulate the spatial distribution of utilization for healthcare resources, based on the Two-Step Floating Catchment Area (2SFCA) method, which is widely used to assess potential spatial accessibility. Instead of using individual-level healthcare utilization data which is difficult to obtain, the EPHI uses institution-level aggregated data, including numbers of inpatient/outpatient visits. By comparing the estimated utilization (based on local healthcare institution services provision) with the expected utilization (based on local population morbidity), guest patients (e.g., patients flowing in for treatment) and bypass patients (patients flowing out) can be identified. To test the applicability of this index, a case study was carried out on China's Hainan Island. The spatial tendencies of patients for inpatient and outpatient services were simulated, then incorporated with spatial access to healthcare resources to evaluate overall resource allocation efficiency, thus guiding future resource allocations and investment for policy makers and healthcare providers. Results: The EPHI revealed that bypass activities widely exist on Hainan Island in both inpatient and outpatient care, with patients tending to travel from less developed regions with fewer healthcare resources to more highly developed regions with more healthcare resources to receive healthcare. Comparison with spatial accessibility demonstrated how bypass activities on Hainan produced an under-utilization of doctors in less developed regions and over-utilization of doctors in more developed coastal regions. Conclusions: This case study on Hainan Island demonstrates that this new index can very clearly identify both the sources and sinks of patient spatial tendencies. Combining these results with spatial accessibility of healthcare resources, how efficiently the available supply matches the utilization can be revealed, indicating wide-ranging applicability for local governments and policymakers.

Biogenic volatile organic compound emission patterns and secondary pollutant formation potentials of dominant greening trees in Chengdu, southwest China.

Integral to the urban ecosystem, greening trees provide many ecological benefits, but the active biogenic volatile organic compounds (BVOCs) they release contribute to the production of ozone and secondary organic aerosols, which harm ambient air quality. It is, therefore, necessary to understand the BVOC emission characteristics of dominant greening tree species and their relative contribution to secondary pollutants in various urban contexts. Consequently, this study utilized a dynamic enclosure system to collect BVOC samples of seven dominant greening tree species in urban Chengdu, Southwest China. Gas chromatography/mass spectrometry was used to analyze the BVOC components and standardized BVOC emission rates of each tree species were then calculated to assess their relative potential to form secondary pollutants. We found obvious differences in the composition of BVOCs emitted by each species. Ficus virens displayed a high isoprene emission rate at 31.472 µgC/(gdw (g dry weight)•hr), while Cinnamomum camphora emitted high volumes of D-Limonene at 93.574 µgC/(gdw•hr). In terms of the BVOC emission rates by leaf area, C. camphora had the highest emission rate of total BVOCs at 13,782.59 µgC/(m2•hr), followed by Cedrus deodara with 5466.86 µgC/(m2•hr). Ginkgo biloba and Osmanthus fragrans mainly emitted oxygenated VOCs with lower overall emission rates. The high BVOC emitters like F. virens, C. camphora, and Magnolia grandiflora have high potential for significantly contributing to environmental secondary pollutants, so should be cautiously considered for future planting. This study provides important implications for improving urban greening efforts for subtropical Chinese urban contexts, like Chengdu.

Evaluation of NOx emissions before, during, and after the COVID-19 lockdowns in China: A comparison of meteorological normalization methods.

Meteorological normalization refers to the removal of meteorological effects on air pollutant concentrations for evaluating emission changes. There currently exist various meteorological normalization methods, yielding inconsistent results. This study aims to identify the state-of-the-art method of meteorological normalization for characterizing the spatiotemporal variation of NOx emissions caused by the COVID-19 pandemic in China. We obtained the hourly data of NO2 concentrations and meteorological conditions for 337 cities in China from January 1, 2019, to December 31, 2020. Three random-forest based meteorological normalization methods were compared, including (1) the method that only resamples meteorological variables, (2) the method that resamples meteorological and temporal variables, and (3) the method that does not need resampling, denoted as Resample-M, Resample-M&T, and Resample-None, respectively. The comparison results show that Resample-M&T considerably underestimated the emission reduction of NOx during the lockdowns, Resample-None generates widely fluctuating estimates that blur the emission recovery trend during work resumption, and Resample-M clearly delineates the emission changes over the entire period. Based on the Resample-M results, the maximum emission reduction occurred during January to February 2020, for most cities, with an average decrease of 19.1 ± 9.4% compared to 2019. During April of 2020 when work resumption initiated to the end of 2020, the emissions rapidly bounced back for most cities, with an average increase of 12.6 ± 15.8% relative to those during the strict lockdowns. Consequently, we recommend using Resample-M for meteorological normalization, and the normalized NO2 concentration dynamics for each city provide important implications for future emission reduction.

Biosorption of V(V) onto Lantana camara biochar modified by H3PO4: Characteristics, mechanism, and regenerative capacity.

Biochar has been widely recognized as an environmentally efficient adsorbent for removing heavy metals. However, considering the weak adsorption performance of the original biochar to the oxygen-containing anion, the adsorption of vanadium by biochar has rarely been investigated. This study proposes that H3PO4 activated biochar made from an invasive plant species growing near mines is a novel material to be investigated for V(V) recovery and reuse. As a noxious, invasive plant, Lantana camara L. (LC) has become widely naturalized around the world. Biochar was prepared from LC by pyrolysis at different conditions (200 °C, 350 °C, 500 °C, and 650 °C). The adsorption effect of biochar with and without P pretreatment on V(V) in aqueous solution was compared. The results show that biochar prepared from LC impregnated with H3PO4 (MLBC) had the highest adsorption capacity at 500 °C, and the maximal adsorption capacity fitted by Langmuir model was 77.38 mg g-1, which was considerably higher than that of untreated biochar (LBC, 5.89 mg g-1). The adsorption procedure was substantially fitted by the Langmuir isotherm and the pseudo-second-order kinetic. Additionally, the interaction of V(V) on MLBC is pH-dependent, and slightly acidic conditions are more favorable for adsorption. The characterization results indicated that electrostatic interaction, complexation reaction, and redox reaction were the primary mechanisms. After three cycles of adsorption, the final maximal adsorption capacity of MLBC remained at 76.03% of that of the virgin sample, demonstrating that MLBC had a recyclable capability to eliminate and restore V(V) from aqueous solutions.

An integrated analysis of spatial access to the three-tier healthcare delivery system in China: a case study of Hainan Island.

BACKGROUND: Access to healthcare is critical for the implementation of Universal Health Coverage. With the development of healthcare insurance systems around the world, spatial impedance to healthcare institutions has attracted increasing attention. However, most spatial access methodologies have been developed in Western countries, whose healthcare systems are different from those in Low- and Middle-Income Countries (LMICs). METHODS: Hainan Island was taken as an example to explore the utilization of modern spatial access techniques under China's specialized Three-Tier Health Care Delivery System. Healthcare institutions were first classified according to the three tiers. Then shortest travel time was calculated for each institution's tier, overlapped to identify eight types of multilevel healthcare access zones. Spatial access to doctors based on the Enhanced Two-Step Floating Catchment Area Method was also calculated. RESULTS: On Hainan Island, about 90% of the population lived within a 60-min service range for Tier 3 (hospital) healthcare institutions, 80% lived within 30 min of Tier 2 (health centers), and 75% lived within 15 min of Tier 1 (clinics). Based on local policy, 76.36% of the population living in 48.52% of the area were able to receive timely services at all tiers of healthcare institutions. The weighted average access to doctors was 2.31 per thousand residents, but the regional disparity was large, with 64.66% being contributed by Tier 3 healthcare institutions. CONCLUSION: Spatial access to healthcare institutions on Hainan Island was generally good according to travel time and general abundance of doctors, but inequity between regions and imbalance between different healthcare institution tiers exist. Primary healthcare institutions, especially in Tier 2, should be strengthened.

Ambient air pollution and male fecundity: A retrospective analysis of longitudinal data from a Chinese human sperm bank (2013-2018).

Ambient air pollution has adverse effects on human health and reproduction. A number of studies have suggested a significant association between ambient air pollution and human fecundity, with most studies focusing on cross-sectional data from the general male population with single semen samples. We conducted a retrospective study in China using longitudinal analysis of repeated semen samples to investigate the association between environmental exposure parameters (e.g., PM2.5, PM10, SO2, NO2, CO, ground temperature, and relative humidity) and sperm quality parameters (e.g., semen volume, sperm concentration, forward motility concentration, and percentage of progressive rate) during different exposure windows (current day, 90-day preceding). Data from 686 males and 4841 semen samples collected between 2013 and 2018 at Sichuan Provincial Sperm Bank were included in a mixed-effects model analysis. The study population was young, healthy, and well-educated. The results indicate that 90-day average concentrations of PM2.5, PM10, and CO were negatively associated with sperm concentration and forward motility concentration, whereas 90-day average concentration of O3 was positively associated with forward motility concentration. Between-subject variability played the dominant role in overall model variance. Our results suggest that chronic exposure to particulate matter and CO may interfere with spermatogenesis.

The combined effects of Cu and Pb on the sex-specific growth and physiology of the dioecious Populus yunnanensis.

In recent years, pollution by heavy metals (HM) has become an increasingly serious problem in forest ecosystems, making their remediation a primary research focus in China. Poplars are ideal candidates for phytoremediation because of their great commercial value, ability to produce large biomass, and high capacity for HM uptake. The individual and combined effects of copper (Cu) and lead (Pb) on Populus yunnanensis growth and physiology were tested for both male and female potted plants in four treatment groups: control, Pb only (1,000 mg kg-1 PbAc dry soil), Cu only (400 mg kg-1 CuSO4·5H2O dry soil), and combined Pb and Cu. Each treatment group contained 25 male and 25 female individuals. The experimental duration was 3 months. Compared with the control plants, the Cu and Pb treatment groups experienced reduced leaf, stem, root, and total biomass for both sexes, but the impact on growth rate was more severe in females than in males. The cellular ultrastructure of leaves was extensively damaged in both male and female trees but was more severely damaged in females. Male trees demonstrated a stronger Cu absorption ability with a bioconcentration factor 2.30 times that of females. Significant changes in pigment content, membrane lipid peroxidation, and protein oxidation (carbonyl) also indicated that females were more sensitive than males to Cu- and Pb-induced stress. The higher Cu and Pb tolerance in males correlated with better H2O2 scavenging ability and proline accumulation. Nevertheless, the combined stress from both Cu and Pb yielded greater negative effect on the growth and physiology of P. yunnanensis for both sexes.

An ethnobotanical survey of wild edible plants used by the Yi people of Liangshan Prefecture, Sichuan Province, China.

BACKGROUND: Due to historical perceptions of Liangshan Yi Autonomous Prefecture (Sichuan Province, China) as being a violent place, and due to its rugged terrain, cultural differences, and relative inaccessibility, few researchers have conducted in-depth ethnobotanical investigations in Liangshan. But wild edible plants (WEPs) are widely consumed by the Yi people of Liangshan, and their associated ethnobotanical knowledge remains relatively unknown, especially outside of China. This study aimed to (1) investigate the WEPs used by the Liangshan Yi, (2) document the traditional knowledge held about these plants, (3) analyze their special preparation methods and consumption habits, and (4) identify species with important cultural significance to the Liangshan Yi. METHODS: During 2016-2017, 396 Yi individuals were interviewed in 1 county-level city and 6 counties across Liangshan. Prior informed consent was obtained, and multiple ethnographic methods were utilized, including direct observation, semi-structured interviews, key informant interviews, informal discussions, and field visits. Market surveys were conducted in April, July, and August 2017 by interviewing 38 Yi merchants selling WEPs in 6 Liangshan traditional markets. We collected information about the parts consumed, preparation methods, consumption habits, growth pattern of species, collection months, market prices, and other uses of WEPs. Use values (UVs) were calculated to analyze the relative cultural importance of each WEP. RESULTS: In total, 105 plant species belonging to 97 genera and 62 families were recorded. Rosaceae was the family with the largest number of species (14), and herbs (58 species) were the dominant growth form reported. Fruits (34 species), roots (21 species), and tender shoots (20 species) were the primary plant parts used for snacking and cooking. There were 6 main preparation and consumption methods of WEPs reported, ranging from primary food, famine food, snack, spice, culinary coagulant, and medicine, among a few other uses. The Liangshan Yi mainly collect WEPs from March to October, seldom collecting from November to February. There were 35 species of WEPs sold in the markets we visited in Liangshan. The price of medicinal plants was much higher than the price of food and fruits. In total, we documented 49 species of edible medicinal plants in Liangshan, accounting for 44.7% of all WEPs. They can be used for treating 27 medical conditions, including cough, diarrhea, injury, and headaches. The plants with the highest UVs were Berberis jamesiana (1.92), Pyracantha fortuneana (1.87), and Artemisia capillaris (1.44) indicating that these species are the most commonly used and important to the Liangshan Yi's traditional life and culture. CONCLUSIONS: The traditional knowledge of WEPs from the accumulated experience of the Yi people's long period residing in Liangshan reflects the cultural richness of the Yi and the plant diversity of the region. Future research on the nutrition, chemical composition, and bioactivity of the WEPs are needed. Some species with high medicinal value but with sharp wild population decline should be surveyed for resource assessment, conservation, and domestication potential.

Air pollution reduction in China: Recent success but great challenge for the future.

China's rapid economic growth has caused severe air pollution, raising serious concerns about the growing evidence of its negative health, environmental, and economic impacts. Consequently, the Chinese government has implemented a number of policies and measures to reduce air pollution. Relying on published information over the last three decades in China, we analyzed trends in air pollutant emissions (SO2 and NOx) and concentrations of particulate matter (PM) and ozone (O3). During the past decade, SO2 and NOx emissions had declined throughout China and concentrations of PM2.5 and PM10 had considerably decreased in most cities, but average reported 90th MDA8 O3, M7, and AOT40 O3 for 31 capital cities showed an increasing trend between 2013 and 2017. Despite progress in air pollution reduction and an increasing number of "clear sky" days, PM concentrations throughout China remain higher than the World Health Organization guidelines, and urban smog and haze remain a major threat to human health and the environment. Thus far, significant emission reductions have occurred largely through robust administrative power, especially when emission reductions were tied to the performance evaluations and promotion of government officials. Similar to most already-industrialized nations, China is now shifting away from SO2-dominated to NOx- and O3-dominated air pollution. Existing technologies and improved operations of existing control equipment appear unlikely to achieve sufficient reductions in NOx and O3 pollution. Considering the complex relationship between O3, NOx, VOCs, weather, and socio-economic changes in China, it is necessary to increase research on impacts of increasing ozone on plants and to adopt novel technologies and implemented to further reduce air pollution to levels that will protect human health and the environment.

A new set of microsatellite primers for Coelogyne fimbriata (Orchidaceae) and cross-amplification in C. ovalis.

PREMISE OF THE STUDY: Declining orchid populations have made it necessary to prioritize the study of population structure and genetic diversity for species including Coelogyne fimbriata (Orchidaceae). METHODS AND RESULTS: A biotin-streptavidin capture method was used to construct a microsatellite library for C. fimbriata. A total of 15 polymorphic nuclear microsatellite loci were isolated and characterized using 47 C. fimbriata individuals from two natural populations in China. The number of alleles per locus for the two populations ranged from two to 17. The observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.000 to 0.867, respectively. Among these polymorphic primers, 11 loci were also successfully amplified in C. ovalis, and 10 loci showed moderate to high-level polymorphism. Cross-amplification of the 15 polymorphic loci was tested in five related species: C. cumingii, C. eberhardtii, C. mayeriana, C. peltastes, and C. velutina. CONCLUSIONS: Fifteen microsatellites in C. fimbriata and 10 in C. ovalis have moderate to high-level genetic variation, indicating their utility in population genetic studies, thus contributing to orchid conservation.